Chiang Mai Journal of Science

Print ISSN: 0125-2526 | eISSN : 2465-3845

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Efficacy of Solid Formulation of Trichoderma virens in Managing Cowpea Crown Rot Caused by Agroathelia rolfsii (Sacc.)

Gannile Herath Mudiyanselage Gedara Sajini Wasundara Nawarathna, Tikka Dewage Chamarika Priyadarshani, Weerawickramage Chaminda Priyanka Egodawatta, Garumuni Dilrukshi Nadeeshani Manike, Entaj Tarafder, Abdallah M. Elgorban, Wenhua Lu and Samantha C. Karunarathna
* Author for corresponding; e-mail address: cpchamarika@agri.rjt.ac.lk, samanthakarunarathna@gmail.com
Volume: Vol.52 No.5 (September 2025)
Research Article
DOI: https://doi.org/10.12982/CMJS.2025.077
Received: 21 May 2025, Revised: 23 July 2025, Accepted: 15 August 2025, Published: 3 September 2025

Citation: Nawarathna G.H.M.G.S.W., Priyadarshani T.D.C., Egodawatta W.C.P., Manike G.D.N., Tarafder E., Elgorban A.M., et al., Efficacy of solid formulation of Trichoderma virens in managing cowpea crown rot caused by Agroathelia rolfsii (Sacc.). Chiang Mai Journal of Science, 2025; 52(5): e2025077. DOI 10.12982/CMJS.2025.077.

Abstract

     This study evaluated the efficacy of Trichoderma virens in controlling Agroathelia rolfsii, the causal agent of cowpea crown rot disease through in vitro, in vivo experiments. Following isolation and confirmation of the pathogen, dual plate assay was conducted in vitro. Pot and field trials were conducted under shade house and in both sterilized and non-sterilized field conditions. Agroathelia rolfsii was inoculated onto the crown area of cowpea seedlings using agar disc method, with one agar disc per plant. Trichoderma virens was applied as a spore suspension at planting and three-week intervals. Plant growth parameters, yield components, CFU, disease incidence were monitored throughout the study. According to the results, morphological, molecular identification confirmed the pathogen as A. rolfsii. Pathogenicity was verified through Koch’s postulate. In the dual plate assay, T. virens inhibited A. rolfsii by 86.02%. The application of T. virens significantly improved plant height, leaf number, flower number, pod number, root length, and seed weight, resulting in zero disease incidence. Furthermore, viability of T. virens in soil was ensured by showing fluctuations in CFU throughout the experimental period. In conclusion, T. virens exhibits significant potential as a biological alternative to chemical fungicides for controlling A. rolfsii, while enhancing cowpea growth.

Keywords: biological control, molecular, morphological, pathogenicity

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