Chiang Mai Journal of Science

Print ISSN: 0125-2526 | eISSN : 2465-3845

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Bacterial Diversity and Pathogenicity of Pine Wood Nematode (Bursaphelenchus xylophilus) from Two Different Sources

Wanyu Dong, Guicai Du, Tingting Zhang, Hong Yang, Qunqun Guo and Ronggui Li
* Author for corresponding; e-mail address: gqunquni@163.com, lrg@qdu.edu.cn
Volume: Vol.49 No.2 (March 2022)
Research Article
DOI: https://doi.org/10.12982/CMJS.2022.024
Received: 19 June 2021, Revised: 8 November 2021, Accepted: 23 November 2021, Published: -

Citation: Dong W., Du G., Zhang T., Yang H., Guo Q. and Li R., Bacterial Diversity and Pathogenicity of Pine Wood Nematode (Bursaphelenchus xylophilus) from Two Different Sources, Chiang Mai Journal of Science, 2022; 49(2): 284-298. DOI 10.12982/CMJS.2022.024.

Abstract

     Pine wilt disease is a devastating disease of pine trees caused by a pine wood nematode (PWN), Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle. We studied the bacteria carried by B. xylophilus from Fushan Mountain and Qingdao University Campus (F and Q samples) and the pathogenicity of the two PWN samples to explore the relationship between PWN companion bacteria and its pathogenicity. Based on metagenomic analysis, the distribution of the bacteria from the two samples was signifi cantly different. At the phylum level, Proteobacteria is the largest phylum in the two samples, 85 and 65% in F and Q samples, respectively. At the family level, Enterobacteriaceae and Rhizobiaceae were dominant in F sample with the total relative abundance of 55% while the two bacterial families only accounted for 15% in Q sample. Moreover, Burkholderiaceae was only detected in F sample. PICRUSt analysis based on KEGG database showed that the bacteria carried by B. xylophilus from F sample had stronger metabolic abilities. In addition, BugBase analysis predicted that the bacteria from F sample had stronger effect on pathogenicity and antioxidant stress. Furthermore, we tested the feeding ability and pathogenicity of F and Q samples, and found that the B. xylophilus of F sample had stronger feeding ability and pathogenicity, which was consistent with the results of PICRUSt analysis based on KEGG database and BugBase analysis. Our research indicated that the pathogenicity of B. xylophilus could be closely related to the vitality of its associated bacteria, and Enterobacteriaceae, Rhizobiaceae and Burkholderiaceae belonging to Proteobacteria phylum were important pathogenicity-related bacterial families, which is valuable for the study on the pathogenesis of B. xylophilus.

Keywords: bacteria, BugBase analysis, Bursaphelenchus xylophilus, metagenomics, pathogenicity, PICRUSt analysis

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Naveen Polkam, Parsharamulu Rayam, Tejeswara Rao Allaka, Shankaraiah Malthum, Naveen Kuntala, Bhaska

Vol.44 No.4 (October 2017)
Research Article View: 1,064 Download: 269
Production of Chitooligosaccharides with Antibacterial Potential via Crude Chitinase Enzymes from Marine Fungi
page: 1224 - 1230

Apiradee Pilantanapak*, Yaowapha Waiprib, Phattharawadee Eadtem and Watanalai Panbangred

Vol.44 No.4 (October 2017)
Research Article View: 1,053 Download: 316
Antibiotics Constituents of Endophytic Bacillus amyloliquefaciens UD25 Extracted from a Medicinal Plant, Memecylon edule Roxb.
page: 788 - 799

Artidtaya Bhoonobtong, Sirirath Sodngam, Sophon Boonlue, Wandee Bunyatratchata and Wiyada Mongkolt

Vol.44 No.3 (July 2017)
Research Article View: 2,223 Download: 1,044
The Establishment and Activity of Bacterial Inoculant Immobilized in Agar Compared with Those of Alginate and Perlite After Being Introduced Into Soil
page: 751 - 767

Pongrawee Nimnoi*, Neelawan Pongsilp and Saisamorn Lumyong

Vol.44 No.3 (July 2017)
Research Article View: 1,025 Download: 359
Detection of Bacterial Communities in Volatile-organic-compound (VOC)-contaminated Soil in an Industrial Estate in Eastern Thailand by PCR-DGGE Analysis
page: 742 - 750

Manatsawat Sriwichai, Fairda Malem, Mujalin K. Pholchan and Sakunnee Bovonsombut*

Vol.44 No.3 (July 2017)
Research Article View: 1,028 Download: 305
Bioactivity, Cytotoxicity and Antibacterial Evaluation of Undoped, Zn-doped, Sr-doped, and Zn/Sr-codoped Hydroxyapatites Synthesized by a Sol-gel Method
page: 630 - 639

Likit Temprom [a], Suphasinee L. Seet [a,b,c], Patcharaporn Tippayawat [d], Pimsiree Suwanna* [a,b,

Vol.44 No.2 (April 2017)
Research Article View: 1,087 Download: 270
Production and Characterization of Bacteriocin-producing Bacteria Capable of Inhibition of Streptococcus dysgalactiae
page: 366 - 374

Nalin Wongkattiya [a], Ian H. Fraser [b], Piyanuch Niamsup [a], Phanchana Sanguansermsri [c] and Don

Vol.44 No.2 (April 2017)
Research Article View: 1,038 Download: 459
Mycorrhizal Spores Associated Lysobacter soli and Its Plant Growth Promoting Activity
page: 94 - 101

Krisana Lasudee, Shinji Tokuyama, Saisamorn Lumyong and Wasu Pathom-aree

Vol.44 No.1 (JANUARY 2017)
Research Article View: 1,011 Download: 371
Rhizobacterial Candidates Isolated from Jerusalem Artichoke (Helianthus tuberosus L.) Rhizosphere for Host Plant Growth Promotion
page: 83 - 93

Kamon Sritongon [a,b], Wiyada Mongkolthanaruk [b], Sophon Boonlue [b], Sanun Jogloy [c,d], Darunee P

Vol.44 No.1 (JANUARY 2017)
Research Article View: 1,004 Download: 351
Production of Plant Growth Promoting Antagonistic Rhizobacteria to Promote Cucumber Growth and Control Leaf Spot Disease (Corynespora cassiicola)
page: 72 - 82

Nuntavun Riddech[a]*, Kamon Sritongon[b]and Thanaporn Phibunwatthanawong[b]

Vol.44 No.1 (JANUARY 2017)
Research Article View: 954 Download: 375
16S rRNA Gene Sequencing Assessment of the Prokaryotic Communities in the Southeast Andaman Sea, Thailand and Potential Environmental Alerts
page: 15 - 28

Khunnalack Khitmoh, Alisa Wilantho, Doonyapong Wongsawaeng, Sissades Tongsima, Naraporn Somboonna*

Vol.44 No.1 (JANUARY 2017)
Research Article View: 997 Download: 277
Antibacterial Activity Against Acne Involved Bacteria of Chitosan in a Soluble State and as Nanoparticles
page: 1149 - 1158

Supreedee Sungkharak [a, b], Nicha Supasit [b], Sasiluk Choopan [b] and Suwipa Ungphaiboon*[a, b]

Vol.43 No.5 (OCTOBER 2016)
Research Article View: 1,127 Download: 773
Encapsulation of Artocarpus lacucha Roxb. Extract in Alginate Chitosan Nanoparticles for Inhibition of Methicillin Resistant Staphylococcus aureus and Bacteria Causing Skin Diseases
page: 946 - 958

Thida Kaewkod [a], Khajornsak Tragoolpua [b] and Yingmanee Tragoolpua* [a]

Vol.43 No.5 (OCTOBER 2016)
Research Article View: 957 Download: 397
Isolation and Screening of Lactobacillus plantarum Strains from Attieke Fermentation Process for A Starter Culture Development
page: 699 - 711

Krabi Ekoua Regina[a], Assamoi Allah Antoine*[a], Ehon Ayawovi Fafadzi[a], Brehima Diawara [b], Niamke Lamine Sebastien [a] and Thonart Philippe [c]

Vol.43 No.4 (JULY 2016)
Research Article View: 1,093 Download: 464
Essential Oil of Solanum spirale Fruits and Its Biological Activities
page: 547 - 555

Sukanya Keawsa-ard [a], Boonsom Liawruangrath* [b], Saisunee Liawruangrath [c], Aphiwat Teerawutgulrag [c] Stephen G. Pyne [d]

Vol.43 No.3 (APRIL 2016)
Research Article View: 1,566 Download: 346
Helvolic Acid, A Secondary Metabolite Produced by Neosartorya spinosa KKU-1NK1 and Its Biological Activities
page: 484 - 494

Wareerat Sanmanoch [a], Wiyada Mongkolthanaruk [a], Somdej Kanokmedhakul [b], Tadanori Aimi [c] and Sophon Boonlue *[a, b]

Vol.43 No.3 (APRIL 2016)
Research Article View: 990 Download: 378
Diversity of Bacteria Nodulating Medicago arborea in the Northeast Area of Morocco
page: 441 - 452

Kamal Guerrouj* [a], Mustapha Bouterfas[b], Hanaa Abdelmoumen [c] and Mustapha Missbah El Idrissi [c]

Vol.43 No.3 (APRIL 2016)
Research Article View: 1,412 Download: 279
Earthy-musty Odour and Off-flavour Taints in Phayao Lake, Thailand
page: 22 - 31

Supannee Suwanpakdee, Redel Gutierrez, Santiwat Pithakpol, Arunothai Jampeetong, Wasu Pathom-aree, Nakao Nomura, Tomoaki Itayama[f] and Niwooti Whangchai

Vol.43 No.1 (JANUARY 2016)
Research Article View: 1,012 Download: 398
Phylogenetic Study of the Botryosphaeriaceae Species Associated with Avocado and Pará rubber in Thailand.
page: 104 - 116

Trakunyingcharoen T., Cheewangkoon R. and To-anun C.

Vol.42 No.1 (JANUARY 2015)
Research Article View: 1,564 Download: 296
Bioactive compounds from Mesua ferrea stems
page: 185 - 195

Sukanya Keawsa-ard and Boonsom Liawruangrath

Vol.42 No.1 (JANUARY 2015)
Research Article View: 1,006 Download: 321
Antimicrobial and antioxidant activity of various solvent extracts of Salsola stenoptera Wagenitz and Petrosimonia nigdeensis Aellen (Chenopodiaceae) plants
page: 156 - 172

Meltem Asan Ozusaglam, Mahmut Erzengin, Derya Onal Darilmaz, Seher Karaman Erkul, Mehtap Tekse

Vol.42 No.1 (JANUARY 2015)
Research Article View: 1,140 Download: 246
Inhibition of Seedling Growth in Giant Mimosa and Reduction of Mitotic Activity in Onion Root Tips Caused by Cyanobacterial Extracts
page: 1150 - 1156

Siriphan Sukkhaeng, Nuttha Sanevas, Srisom Suwanwong*

Vol.41 No.5/1 (OCTOBER 2014)
Research Article View: 974 Download: 408
Culturable Bacterial Community in a High Temperature EBPR Reactor
page: 970 - 980

Ying Hui Ong 1a, Adeline Seak May Chua 2a*, Geok Yuan Annie Tan3b, and Gek Cheng Ngoh 4a

Vol.41 No.5/1 (OCTOBER 2014)
Research Article View: 1,035 Download: 383
Synthesis, Characterization and Antibacterial Evaluation of Some Mixed-metal Mixed-ligand Complexes
page: 625 - 635

Mohammad N. Uddin*, Didarul A. Chowdhury, Jahwa Islam

Vol.40 No.4 (OCTOBER 2013)
Research Article View: 982 Download: 279
Antibacterial Activity and Chemical Composition of Essential Oil and Various Extracts of Fagraea fragrans Roxb. Flowers
page: 214 - 223

Patcharee Pripdeevech* [a] and Jarupux Saansoomchai [b]

Vol.40 No.2 (APRIL 2013)
Research Article View: 1,161 Download: 519
Characteristics of Phosphate Solubilization by Phosphate-Solubilizing Bacteria Isolated from Agricultural Chili Soil and Their Efficiency on the Growth of Chili (Capsicum frutescens L. cv. Hua Rua)
page: 11 - 25

Worapon Surapat[a], Charida Pukahuta[a], Pongsak Rattanachaikunsopon[a], Tadanori Aimi[b], and Sopho

Vol.40 No.1 (JANUARY 2013)
Research Article View: 911 Download: 303
Evaluation of Effective Entomopathogenic Fungi to Fruit Fly Pupa, Bactrocera spp., and Their Antimicrobial Activity
page: 464 - 477

Thet Thet Mar and Saisamorn Lumyong*

Vol.39 No.3 (JULY 2012)
Research Article View: 940 Download: 293
Antioxidant, Antibacterial, Anticancer Activities and Chemical Constituents of the Essential Oil from Mesua ferrea Leaves
page: 455 - 463

Sukanya Keawsa-ard* [a] and Samart Kongtaweelert [b]

Vol.39 No.3 (JULY 2012)
Research Article View: 1,115 Download: 626
Anticancer and Antibacterial Activities of the Isolated Compounds from Solanum spirale Roxb. Leaves
page: 445 - 454

Sukanya Keawsa-ard [a], Surapol Natakankitkul [a], Saisunee Liawruangrath [b], Aphiwat Teerawutgulra

Vol.39 No.3 (JULY 2012)
Research Article View: 1,056 Download: 414
Antibacterial Activity of Bauhinia acuminata L. Seed Protein Extract with Low Hemolytic Activity Against Human Erythrocytes
page: 242 - 251

Karaket Phansri [a], Rakrudee Sarnthima [a], Sompong Thammasirirak [b], Pasakorn Boonchalee [c] and

Vol.38 No.2 (APRIL 2011)
Research Article View: 1,088 Download: 277
Genetical Diversity of Mastigocladus in Ranong Hot Spring, Southern Part of Thailand
page: 363 - 370

Udomluk Sompong* [a], Richard W. Castenholz [b], Somboon Anuntalabhochai [a] and Yuwadee Peerapornp

Vol.33 No.3 (SEPTEMBER 2006)
Research Article View: 1,520 Download: 439
Growth Inhibition of Ralstonia solanacearum PT1J by Antagonistic Bacteria Isolated from Soils in the Northern Part of Thailand
page: 345 - 354

Narumol Thongwai and Jirapa Kunopakarn

Vol.34 No.3 (SEPTEMBER 2007)
Research Article View: 1,047 Download: 452
L-Sorbose Production by Acidotolerant Acetic Acid Bacteria Isolated from Thailand Sources
page: 382 - 390

Watcharee Hanmoungjai, Ekachai Chukeatirote,Yuzo Yamada, Verasak Sahachaisaree, Pipob Lumyong , Gor

Vol.35 No.2 (MAY 2008)
Research Article View: 1,005 Download: 260
Hepatotoxin Biosynthesis and Regulation in Cyanobacteria – the Putative Involvement of Nitrogen and Iron Homeostasis Mechanisms
page: 200 - 223

Hannah P. Ginn, Leanne A. Pearson and Brett A. Neilan

Vol.36 No.2 (MAY 2009)
Short Communication View: 1,025 Download: 254
Characterization of Various Proteases from SaltTTTolerant Bacteria Isolated from Thai Fermented Foods Foods
page: 149 - 161

Somkid Deejing* [a,b], Mitsuaki Moriguchi [c], Naiyatat Poosaran [d], Griangsak Chairote [e] and Sa

Vol.32 No.2 (MAY 2005)
Research Article View: 1,526 Download: 262
Selective Isolation of Sorbitol Utilizing Acetic Acid Bacteria
page: 189 - 192

Jeerapun Jaiinphon, Kreuawan Thonglem and Wasu Pathom-aree*

Vol.29 No.3 (DECEMBER 2002)
Research Article View: 1,055 Download: 265
Survey and Monitoring of Toxic Cyanobacteria in Water Resources for Water Supplies and Fisheries in Thailand
page: 71 - 79

Yuwadee Peerapornpisal*, Wanchai Sonthichai, Morakot Sukchotiratana, Suwiwek Lipigorngoson, Werawan Ruangyuttikarn, Komson Ruangrit, Jeeraporn Pekkoh, Rattapoom Prommana, Nopparut Panuvanitchakorn, Neti Ngearnpat, Siripong Kiatpradab, Sakorn Promkutkaew

Vol.29 No.2 (AUGUST 2002)
Opinion View: 1,062 Download: 340
Inhibitory Effect of Thermotolerant Lactic Acid Bacteria on the Growth of Bacillus cereus, Staphylococcus aureus, Salmonella enteritidis and Vibrio parahaemolyticus
page: 119 - 124

Atchara Lapmak [a], Sumana Kumpai [b] and Abhinya Plikomol*[b]

Vol.28 No.2 (DECEMBER 2001)
Opinion View: 1,111 Download: 278
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