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Optimization of GABA Production and Partial gad Gene Identification of Potential Probiotic Levilactobacillus brevis F064A Isolated from Thai Fermented Sausage

Paper Type 
Contributed Paper
Optimization of GABA Production and Partial gad Gene Identification of Potential Probiotic Levilactobacillus brevis F064A Isolated from Thai Fermented Sausage
Jirapat Kanklai, Narumol Thongwai, Patthanasak Rungsirivanich, Yingmanee Tragoolpua and Jeeraporn Pekkoh

     Gamma-aminobutyric acid (GABA), an inhibitory neurotransmitter, can be produced by lactic acid bacteria. Levilactobacillus brevis F064A, a high potential probiotic, is capable of producing GABA. To elucidate its glutamic acid decarboxylase (GAD) enzyme responsible for GABA production, partial gad genes were examined. It was found that both partial gadA and gadB genes were existing in L. brevis F064A genomes. Some infl uencing effects on GABA production by Levilactobacillus brevis F064A were determined. The highest GABA production of 4.18 ± 0.07 mg/mL was achieved with the optimal conditions of initial media pH of 5.5, 2% (w/v) of glucose, 2% (w/v) of monosodium glutamate (MSG) and 48 h at 37°C of incubation. Pyridoxal 5′-phosphate (PLP) had no effect on its GABA production. At 48 h of incubation, the viable cells were increased as well as the culture pH. Moreover, the optimum conditions based on the response surface methodology (RSM) model were pH of 5.26, 2.71% (w/v) of glucose and 2.96% (w/v) of MSG yielded 4.53 ± 0.08 mg/mL GABA content (p < 0.05). Additionally, total lactic acid of 10.30 ± 0.91 mg/mL was also produced. Promisingly, L. brevis F064A is a good candidate for GABA production and for functional food development in the future.

Start & End Page 
1013 - 1027
Received Date 
Revised Date 
Accepted Date 
Full Text 
lactic acid bacteria, fermentation, GABA, RSM, monosodium glutamate
Vol.49 No.4 (July 2022)
Kanklai J., Thongwai N., Rungsirivanich P., Tragoolpua Y. and Pekkoh J., Optimization of GABA Production and Partial gad Gene Identification of Potential Probiotic Levilactobacillus brevis F064A Isolated from Thai Fermented Sausage, Chiang Mai J. Sci., 2022; 49(4): 1013-1027. DOI 10.12982/CMJS.2022.068.
View:780 Download:374


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Biohydrogen Production by Extremely Halophilic Bacteria from the Salt Pan of Samut Sakhon, Thailand
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Simultaneous Saccharification and Fermentation for Ethanol Production from Rice Straw by Candidashehatae and Saccharomyces cerevisiae
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Vol.45 NO.7 (November 2018) View: 566 Download:188
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Vol.45 No.3 (May 2018) View: 553 Download:164
Improvement of a Continuous Ethanol Fermentation from Sweet Sorghum Stem Juice by Aeration and Product Recovery using a Gas Stripping Technique
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Vol.45 No.3 (May 2018) View: 1,049 Download:213
Production of L-Lactic Acid by Thermotorelant Lactic Acid Bacteria
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Bioconversion of Agricultural Wastes to Mannooligosaccharides and Their Prebiotic Potential
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Vol.45 No.1 (January 2018) View: 587 Download:251
Response Surface Methodology Based Optimized Purification of the Residual Glycerol from Biodiesel Production Process
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Vol.44 No.4 (October 2017) View: 640 Download:313
Influences of Buffer Systems on Enzymatic Saccharification of Rice Husk Holocellulose and Fermenting Ability of Various Ethanol Producing Microorganisms
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Vol.44 No.2 (April 2017) View: 530 Download:138
Production and Characterization of Bacteriocin-producing Bacteria Capable of Inhibition of Streptococcus dysgalactiae
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Author:Nalin Wongkattiya [a], Ian H. Fraser [b], Piyanuch Niamsup [a], Phanchana Sanguansermsri [c] and Don
Vol.44 No.2 (April 2017) View: 563 Download:219
Determination of GABA Content in Thai Brown Rice by an Optimized Enzyme-based Method
page: 132 - 143
Author:Suwapat Kittibunchakul, Parunya Thiyajai, Uthaiwan Suttisansanee and Chalat Santivarangkna*
Vol.44 No.1 (JANUARY 2017) View: 584 Download:525
Utilization of Non-rubber Skim Latex for Poly(L-lactide)-degrading Enzyme Production by Actinomadura keratinilytica Strain T16-1
page: 1016 - 1026
Author:Sabadee Worrathampitak [a], Shinji Tokuyama [b], Vichien Kitpreechavanich [c] and Sukhumaporn Sukkhum* [a]
Vol.43 No.5 (OCTOBER 2016) View: 580 Download:206
Improved SSF-cellulosic ethanol production by the cellobiose fermenting yeast Kluyveromyces marxianus G2-16-1
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Author:Ancharida Akaracharanya*[a], Kridsana Krisomdee [b], Vasana Tolieng [c], Vichien Kitpreechavanich [d] and Somboon Tanasupawat [e]
Vol.43 No.5 (OCTOBER 2016) View: 571 Download:245
Enhanced Production of Cordycepin by Solid State Fermentation of Cordyceps militaris using Additives
page: 972 - 984
Author:Ting-Chi Wen [a]+, Chao Kang [b]+, Ze-Bing Meng [a], Yong-Bin Qi [a], Kevin D. Hyde [c, d] and Ji-Chuan Kang* [a]
Vol.43 No.5 (OCTOBER 2016) View: 735 Download:193
Bio-hydrogen Production from Rabbit Dung by Anaerobic Self-fermentation
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Author:Sucheera Laocharoen [a] and Alissara Reungsang* [a, b]
Vol.43 No.4 (JULY 2016) View: 548 Download:236
Isolation and Screening of Lactobacillus plantarum Strains from Attieke Fermentation Process for A Starter Culture Development
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Author:Krabi Ekoua Regina[a], Assamoi Allah Antoine*[a], Ehon Ayawovi Fafadzi[a], Brehima Diawara [b], Niamke Lamine Sebastien [a] and Thonart Philippe [c]
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Utilization of agro-industrial products for increasing red pigment production of Monascus purpureus AHK12
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Effects of initial glucose concentration on fermentation performance of Kluyveromyces marxianus cells immobilized on banana leaf sheath pieces
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Author:Hoang Du Le, Van Viet Man Le
Vol.42 No.2 (APRIL 2015) View: 553 Download:211
Improvement of continuous ethanol fermentation from sweet sorghum juice by Saccharomyces cerevisiae using stirred tank bioreactor coupling with plug flow bioreactor
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Isolation and study of thermotolerant Bacillus strains including L-lactic acid production from kitchen refuse
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Author:Saowanit Tongpim and Kenji Sakai
Vol.42 No.1 (JANUARY 2015) View: 479 Download:166
Phenolics production from a novel substrate palm oil mill effluent by Aspergillus niger IBS-103ZA: Evaluation of fermentation conditions and antioxidant activity
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Author:Zulkarnain Mohamed Idris, Parveen Jamal, Md Zahangir Alam, Abdul Haseeb Ansari, A
Vol.42 No.1 (JANUARY 2015) View: 544 Download:167
Effect of Fermented Red Dragon Fruit (Hylocereus polyrhizus) Liquid on Cholesterol and Triglycerides Level of Golden Syrian Hamsters
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Author:Foong Jia Huan*, Hon Wei Min and Ho Chun Wai
Vol.41 No.5/1 (OCTOBER 2014) View: 609 Download:260
Optimization of Solid-state Fermentation for Fruiting Body Growth and Cordecepin Production by Cordyceps militaris
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Author:Ting-chi Wen, Cuang-rong Li, Ji-chuan Kang*,Chao Kang and Kevin D. Hyde
Vol.41 No.4 (SPECIAL ISSUE 1) View: 626 Download:476
Phenotypic Marking of Cordyceps militaris Fruiting-Bodies and Their Cordycepin Production
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Author:Ting-chi Wen, Ji-chuan Kang*, Kevin D. Hyde, Guang-rong Li, Chao Kang and Xu Chen
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Review of Angkak Production (Monascus purpureus)
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Screening of Nutrient Parameters for Red Pigment Production by Monascus purpureus MTCC 369 Under Submerged Fermentation Using Plackett- Burman Design
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γ-Aminobutyric Acid (GABA) Accumulations in Rice During Germination
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Vol.37 No.1 (JANUARY 2010) View: 550 Download:160
Inhibitory Effect of Thermotolerant Lactic Acid Bacteria on the Growth of Bacillus cereus, Staphylococcus aureus, Salmonella enteritidis and Vibrio parahaemolyticus
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Vol.28 No.2 (DECEMBER 2001) View: 637 Download:171
Selection of Starter Cultures for the Production of Vegetarian Kapi, a Thai Fermented Condiment
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Vol.37 No.2 (MAY 2010) View: 508 Download:182

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Chiang Mai Journal of Science

Faculty of Science, Chiang Mai University
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