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Identification and Validation of Blast Resistance Loci from the Durable Resistance Cultivar Hahng Yi 71


Paper Type 
Contributed Paper
Title 
Identification and Validation of Blast Resistance Loci from the Durable Resistance Cultivar Hahng Yi 71
Author 
Anucha Plabpla, Siriporn Korinsak, Siripar Korinsak, Burin Thunnom, Apichart Vanavichit, Somjai Saleeto, Kanyanat Sirithanya and Theerayut Toojinda
Email 
theerayut@biotec.or.th
Abstract:

     One of the effective strategies for developing blast disease-resistant cultivars to mitigate the yield losses caused by Pyricularia oryzae is the discovery of new genetic resources containing resistance genes. In the present study, a Thai landrace glutinous rice cultivar Hahng Yi 71 (HY71) was used as a durable, broad resistance source to identify QTLs against blast fungus and crossed with a susceptible recurrent parent cultivar RD6, generating 56 BC4F5 lines. The BC4F5 population was tested with six blast isolates, including THL54, THL583, THL658, THL690, THL795, and THL1119, and evaluated with 161 SSR markers to identify resistance regions using modified bulk segregation analysis. Single marker analysis showed 7 markers linked to a resistance reaction located on chromosomes 2, 4, and 5, then tentatively designated as qBL2-RD6, qBL4-HY71, and qBL5-HY71, respectively. Two introgressed lines from the mapping population, KKN97057-7-1-1-2-5-NKI-4-1 and KKN97057-7-1-1-2-5-NKI-5-1, were the qBL5-HY71 donor for introgressing into two rice lines, RGD07123-MS12-MS22-MS1, and RGD07123-MS12-MS22-MS9, which generated two single crosses and a backcross population by marker-assisted selection (MAS) using the linked markers RM39 and RM164. Three improved lines, RGD10021-MS18-B-MS8-MS40, RGD10031-MS-MS3-B-MS9-MS42 (single cross), and RGD11086-MS-MS15-MS10-MS19 (backcross), carrying qBL5-HY71, were tested for their resistance against leaf and neck blast disease by 62 and 8 blast isolates, respectively. The disease screening results showed that qBL5-HY71 was very effective in controlling leaf and neck blast. This study demonstrates the effectiveness of resistance loci qBL5-HY71 from HY71 on chromosome 5 and their linked markers in improving durable blast resistance in rice breeding programs.

Article ID
e2024070
Received Date 
2024-01-16
Revised Date 
2024-06-29
Accepted Date 
2024-07-01
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Keyword 
rice blast disease, resistance gene, broad-spectrum resistance, marker-assisted selection, Indica rice, Hahng Yi 71
Volume 
Vol.51 No.5 (September 2024)
DOI 
https://doi.org/10.12982/CMJS.2024.070
Citation 
Plabpla A., Korinsak S., Korinsak S., Thunnom B., Vanavichit A., Saleeto S., et al., Identification and Validation of Blast Resistance Loci from the Durable Resistance Cultivar Hahng Yi 71, Chiang Mai J. Sci., 2024; 51(5): e2024070. DOI 10.12982/CMJS.2024.070.
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Chiang Mai Journal of Science

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