Biocatalytic Aldol Addition Reactions by Lipase from Aspergillus oryzae
Hongde Yan* Author for corresponding; e-mail address: yanhd20@126.com
Volume: Vol.52 No.2 (March 2025)
Research Article
DOI: https://doi.org/10.12982/CMJS.2025.019
Received: 21 November 2024, Revised: 11 Febuary 2025, Accepted: 27 Febuary 2025, Published: 24 March 2025
Citation: Yan H., Biocatalytic aldol addition reactions by lipase from Aspergillus oryzae. Chiang Mai Journal of Science, 2025; 52(2): e2025019. DOI 10.12982/CMJS.2025.019.
Graphical Abstract
Abstract
This study explored the catalytic promiscuity of a lipase from Aspergillus oryzae, showing its ability to facilitate an aldol reaction between 4-nitrobenzaldehyde and cyclohexanone. Optimization of conditions— specifically, using acetonitrile as the solvent at 30 °C with 5% water content, a 35:1 molar ratio of cyclohexanone to 4-nitrobenzaldehyde, and an enzyme concentration of 20 mg/mL—led to a 95% conversion, with an 83:17 diastereomeric ratio of the products and 80% enantiomeric excess of anti-products. The findings demonstrated the enzyme’s potential in organic synthesis, expanding its biocatalytic applications.