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Biocatalytic Aldol Addition Reactions by Lipase from Aspergillus oryzae
Paper Type  |
Contributed Paper |
Title |
Biocatalytic Aldol Addition Reactions by Lipase from Aspergillus oryzae |
Author |
Hongde Yan |
Email |
yanhd20@126.com |
|
Abstract: This study explored the catalytic promiscuity of a lipase from Aspergillus oryzae, showing its ability to facilitate an aldol reaction between 4-nitrobenzaldehyde and cyclohexanone. Optimization of conditions— specifically, using acetonitrile as the solvent at 30 °C with 5% water content, a 35:1 molar ratio of cyclohexanone to 4-nitrobenzaldehyde, and an enzyme concentration of 20 mg/mL—led to a 95% conversion, with an 83:17 diastereomeric ratio of the products and 80% enantiomeric excess of anti-products. The findings demonstrated the enzyme’s potential in organic synthesis, expanding its biocatalytic applications. |
|
Graphical Abstract: |
|
Article ID |
e2025019 |
Received Date |
2024-11-21 |
Revised Date |
2025-02-11 |
Accepted Date |
2025-02-27 |
Published Date |
2025-03-24 |
Full Text |
Download |
File Supplemental |
Download Supplemental |
Keyword |
aldol reactions, biocatalyst, catalytic promiscuity, lipase, stereoselectivity |
Volume |
Vol.52 No.2 (March 2025) |
DOI |
https://doi.org/10.12982/CMJS.2025.019 |
Citation |
Yan H., Biocatalytic Aldol Addition Reactions by Lipase from Aspergillus oryzae, Chiang Mai Journal of Science, 2025; 52(2): e2025019. DOI 10.12982/CMJS.2025.019. |
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