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Isolation of Erucic Acid from Mustard Seed Oil by Lipase Hydrolysis and Crystallization
Paper Type  |
Opinion |
Title |
Isolation of Erucic Acid from Mustard Seed Oil by Lipase Hydrolysis and Crystallization |
Author |
Raviwan Tinoi , Hataichanoke Niamsup and Nuansri Rakariyatham* |
Email |
- |
|
Abstract: The isolation of fatty acids from mustard seed oil after lipase hydrolysis was investigated. The mustard seed oil was hydrolyzed by Candida rugosa lipase at room temperature for 20 hours and the methyl ester derivatives of their respective fatty acids were analyzed by gas chromatography. Ten fatty acids were identified, comprising 18.9% by mass of the mustard seed oil. Percent by weight of oleic acid, linoleic acid, linolenic acid and erucic acid were found to be 6.16, 3.35, 1.09 and 4.85 of the mustard seed oil, respectively (or 32.6, 17.7, 5.77 and 25.6 of total fatty acid, respectively). Crystallization of erucic acid from the hydrolysis mixture at -11° C using several solvents (ethanol, methanol or acetone) was investigated. The most suitable solvent was aqueous ethanol ( 8:1, ethanol : water, v/v) which resulted in erucic acid crystallized with 34.0% purity.
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Start & End Page |
89 - 101 |
Received Date |
1999-08-15 |
Revised Date |
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Accepted Date |
2000-09-03 |
Full Text |
Download |
Keyword |
Lipase, erucic acid, mustard seed, brassica juncea |
Volume |
Vol.27 No.2 (DECEMBER 2000) |
DOI |
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Citation |
Tinoi R., Niamsup H. and Rakariyatham* N., Isolation of Erucic Acid from Mustard Seed Oil by Lipase Hydrolysis and Crystallization, Chiang Mai Journal of Science, 2000; 27(2): 89-101. |
| View:1,291 Download:234 | |
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