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Analysis of Intact Glucosinolates in Thai Broccoli Seeds
Paper Type  |
Contributed Paper |
Title |
Analysis of Intact Glucosinolates in Thai Broccoli Seeds |
Author |
Sarunya Chuanphongpanich*[a], Duang Buddhasukh [b], Pichai Pirakitikulr [b] and Sukon Phanichphant |
Email |
sarunyag4470135@yahoo.com |
|
Abstract: This study describes a validated reverse-phase paired-ion chromatography (RP-PIC) with diode array detector method to determine the intact glucosinolates in various Thai broccoli seed genotypes. It is a modified of the official AOCS method and followed the extraction glucosinolates step from broccoli seeds. The analyses were carried out on a Lichrospher RP-18 column (150 mm x 2.1 mm i.d., 5 mm), with a mobile phase gradient, which increases the acetonitrile level in a 50% acetonitrile plus 50% de-ionized water containing 5mM tetraoctylammonium bromide. The flow rate was 0.20 ml/min and detection set at 229 nm. These procedures were applied to samples of broccoli seeds cultivars grown in Thailand, in order to resolve and identify its major glucosinolates: 3-methylsulfinylpropyl-, 4- methylsulfinylbutyl-, 5-methylsulfinylpentyl-, 4-hydroxy-3-indolylmethyl-, 4-methylthiobutyl-, 4-methoxy-3-indolylmethyl- and 1-methoxy-3-indolylmethyl glucosinolate and the detection limits were 0.026 µg/ml, 0.031 µg/ml, 0.025 µg/ml, 0.022 µg/ml, 0.028 µg/ml, 0.021 µg/ml and 0.022 µg/ml, respectively. Thus, this analytical methodology provides a powerful technique for identifying and quantitating glucosinolates in broccoli seeds extracts without derivatization.
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Start & End Page |
223 - 230 |
Received Date |
2005-04-12 |
Revised Date |
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Accepted Date |
2005-08-03 |
Full Text |
Download |
Keyword |
broccoli seeds, cultivar, genotype, glucosinolates, RP-PIC |
Volume |
Vol.33 No.2 (MAY 2006) |
DOI |
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Citation |
Chuanphongpanich*[a] S., [b] D.B., [b] P.P. and Sukon Phanichphant , Analysis of Intact Glucosinolates in Thai Broccoli Seeds, Chiang Mai Journal of Science, 2006; 33(2): 223-230. |
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