Chiang Mai Journal of Science

Print ISSN: 0125-2526 | eISSN : 2465-3845

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Hypoglycemic Activity and Acute Toxicity of Human Insulin-tat Mixture

Aranya Manosroi, Chanutchamon Sutthiwanjampa, Worapaka Manosroi, Rolf G. Werner, Friedrich Götz, Theeraphong Tangjai, Worapong Kitdamrongtham and Jiradej Manosroi
* Author for corresponding; e-mail address: jiradej.manosroi8@gmail.com
Volume: Vol.45 No.1 (January 2018)
Research Article
DOI:
Received: 25 December 2015, Revised: -, Accepted: 1 December 2016, Published: -

Citation: Manosroi A., Sutthiwanjampa C., Manosroi W., Werner R.G., Götz F., Tangjai T., et al., Hypoglycemic Activity and Acute Toxicity of Human Insulin-tat Mixture, Chiang Mai Journal of Science, 2018; 45(1): 198-210.

Abstract

When orally administered in fasted alloxan-induced diabetic mice, human insulin mixed with the cell penetrating peptides, the trans-activator of transcription (Tat) gave the hypoglycemic activity, while mixed with the VP1-BC loop polioviral capsid (VP) showed no hypoglycemic activity. The insulin-Tat mixture (1:3 molar ratio) at the insulin dose of 200 IU/kg body weight showed significant different (p<0.01) in comparing to the control group with the highest percentage reduction of blood glucose level of 77.29±3.77% or 1.53 folds more than the subcutaneously injected insulin in the fasted diabetic mice after 10 hours of administration. Molecular interaction between insulin and Tat was investigated by the reflectometric interference spectroscopy (RIfS). RIfS of insulin-coated transducer chip exhibited the signal of binding or molecular interaction only with Tat, but no signal of binding with BSA and peptide7 (GAVGAVG) which were used as the controls. Acute toxicity studies revealed that insulin-Tat mixture (1:3 molar ratio) gave no toxicity and safe in mice with the LD50 value of more than 2,000 mg/kg body weight. The results from this study can be used for the further development of insulin-Tat as an effective oral insulin delivery.

Keywords: human insulin, Tat, acute toxicity, oral delivery, hypoglycemic activity, VP1-BC loop polioviral capsid

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Antibacterial Activity Against Acne Involved Bacteria of Chitosan in a Soluble State and as Nanoparticles
page: 1149 - 1158

Supreedee Sungkharak [a, b], Nicha Supasit [b], Sasiluk Choopan [b] and Suwipa Ungphaiboon*[a, b]

Vol.43 No.5 (OCTOBER 2016)
Research Article View: 1,127 Download: 773
Dye-sensitized Solar Cell Based on Composite Poly(ethylene oxide) Electrolyte with Natural Carmine Pigment
page: 1113 - 1121

Surassawatee Jamikorn [a], Suthida Vivekaphirat [a], Voranuch Somsongkul [c], Weera Suanthaisong [a], Atittaya Naikaew [b], Atchana Wongchaisuwat [a] and Marisa Arunchaiya*[b]

Vol.43 No.5 (OCTOBER 2016)
Research Article View: 833 Download: 271
Utilization of Non-rubber Skim Latex for Poly(L-lactide)-degrading Enzyme Production by Actinomadura keratinilytica Strain T16-1
page: 1016 - 1026

Sabadee Worrathampitak [a], Shinji Tokuyama [b], Vichien Kitpreechavanich [c] and Sukhumaporn Sukkhum* [a]

Vol.43 No.5 (OCTOBER 2016)
Research Article View: 1,079 Download: 368
Improved SSF-cellulosic ethanol production by the cellobiose fermenting yeast Kluyveromyces marxianus G2-16-1
page: 985 - 996

Ancharida Akaracharanya*[a], Kridsana Krisomdee [b], Vasana Tolieng [c], Vichien Kitpreechavanich [d] and Somboon Tanasupawat [e]

Vol.43 No.5 (OCTOBER 2016)
Research Article View: 1,013 Download: 594
Enhanced Production of Cordycepin by Solid State Fermentation of Cordyceps militaris using Additives
page: 972 - 984

Ting-Chi Wen [a]+, Chao Kang [b]+, Ze-Bing Meng [a], Yong-Bin Qi [a], Kevin D. Hyde [c, d] and Ji-Chuan Kang* [a]

Vol.43 No.5 (OCTOBER 2016)
Research Article View: 1,318 Download: 312
Three Non-parametric Control Charts Based on Ranked Set Sampling
page: 915 - 930

Waliporn Tapang, Adisak Pongpullponsak* and Sukuman Sarikavanij

Vol.43 No.4 (JULY 2016)
Research Article View: 1,452 Download: 424
Bio-hydrogen Production from Rabbit Dung by Anaerobic Self-fermentation
page: 726 - 737

Sucheera Laocharoen [a] and Alissara Reungsang* [a, b]

Vol.43 No.4 (JULY 2016)
Research Article View: 944 Download: 398
Isolation and Screening of Lactobacillus plantarum Strains from Attieke Fermentation Process for A Starter Culture Development
page: 699 - 711

Krabi Ekoua Regina[a], Assamoi Allah Antoine*[a], Ehon Ayawovi Fafadzi[a], Brehima Diawara [b], Niamke Lamine Sebastien [a] and Thonart Philippe [c]

Vol.43 No.4 (JULY 2016)
Research Article View: 1,094 Download: 464
Helvolic Acid, A Secondary Metabolite Produced by Neosartorya spinosa KKU-1NK1 and Its Biological Activities
page: 484 - 494

Wareerat Sanmanoch [a], Wiyada Mongkolthanaruk [a], Somdej Kanokmedhakul [b], Tadanori Aimi [c] and Sophon Boonlue *[a, b]

Vol.43 No.3 (APRIL 2016)
Research Article View: 990 Download: 378
Precipitation in the Nugget Zone of AA6061-T6 by Friction Stir Welding
page: 409 - 419

Wang Tao, Zou Yong, Kenji Matsuda

Vol.43 No.2 (SPECIAL ISSUE 1)
Research Article View: 1,250 Download: 2,010
Semi-Solid State Joining of SSM Aluminum Alloys Using Brazing ZA27
page: 393 - 401

Thammanoon Phonin[a] , Prapas Muangjunburee*[b]

Vol.43 No.2 (SPECIAL ISSUE 1)
Research Article View: 877 Download: 283
Synthesis and characterization of cubic-like zinc stannate powders prepared by co-precipitation method
page: 345 - 350

Wanichaya Mekprasart, Thanit Tangcharoen, Puritat Nakhanivej and Wisanu Pecharapa

Vol.43 No.2 (SPECIAL ISSUE 1)
Research Article View: 871 Download: 213
Electrospun Cellulose Acetate Fiber Mats Containing Emodin with Potential for Use as Wound Dressing
page: 195 - 205

Jate Panichpakdee [a], Prasit Pavasant [b] and Pitt Supaphol* [c,d]

Vol.43 No.1 (JANUARY 2016)
Research Article View: 941 Download: 237
Improvement of production and stability of silk degumming protease by Bacillus sp. C4 SS-2013
page: 599 - 613

Nisa Romsomsa, Patoomporn Chim-anage, Sarote Sirisansaneeyakul

Vol.42 No.3 (JULY 2015)
Research Article View: 1,395 Download: 290
Utilization of agro-industrial products for increasing red pigment production of Monascus purpureus AHK12
page: 331 - 338

Pongrawee Nimnoi , Neelawan Pongsilp, Saisamorn Lumyong

Vol.42 No.2 (APRIL 2015)
Research Article View: 989 Download: 267
Effects of initial glucose concentration on fermentation performance of Kluyveromyces marxianus cells immobilized on banana leaf sheath pieces
page: 294 - 303

Hoang Du Le, Van Viet Man Le

Vol.42 No.2 (APRIL 2015)
Research Article View: 986 Download: 308
Improvement of continuous ethanol fermentation from sweet sorghum juice by Saccharomyces cerevisiae using stirred tank bioreactor coupling with plug flow bioreactor
page: 282 - 293

Naulchan Khongsay, Lakkana Laopaiboon and Pattana Laopaiboon

Vol.42 No.2 (APRIL 2015)
Research Article View: 942 Download: 262
Population Size and Habitat Patterns of Indo-Pacific Humpback Dolphins (Sousa chinensis) off Donsak, Surat Thani, Thailand
page: 136 - 147

Suwat Jutapruet, Kongkiat Kittiwattanawong, Siriporn Pradit

Vol.42 No.1 (JANUARY 2015)
Research Article View: 851 Download: 341
Isolation and study of thermotolerant Bacillus strains including L-lactic acid production from kitchen refuse
page: 62 - 69

Saowanit Tongpim and Kenji Sakai

Vol.42 No.1 (JANUARY 2015)
Research Article View: 927 Download: 309
Spectroscopic electron temperature measurement in methane/helium plasma during diamond-like carbon coating
page: 248 - 257

Artit Chingsungnoen , Vittaya Amornkitbamrung

Vol.42 No.1 (JANUARY 2015)
Research Article View: 1,475 Download: 295
Synthesis, Characterization and Antimicrobial Evaluation of Transition Metal Complexes of Monodentate 2-(Substituted Phenyl)-1H-benzo[d]imidazoles
page: 216 - 227

Minaxi S Maru and Manish K Shah

Vol.42 No.1 (JANUARY 2015)
Research Article View: 1,037 Download: 296
Phenolics production from a novel substrate palm oil mill effluent by Aspergillus niger IBS-103ZA: Evaluation of fermentation conditions and antioxidant activity
page: 1 - 16

Zulkarnain Mohamed Idris, Parveen Jamal, Md Zahangir Alam, Abdul Haseeb Ansari, A

Vol.42 No.1 (JANUARY 2015)
Research Article View: 1,072 Download: 311
Enhanced Production of Polysaccharides and Protein Content in Cyanobacterium, Oscillatoria limnetica as a Defense Mechanism Against Low pH and Pb2+
page: 34 - 43

Suneerat Ruangsomboon*

Vol.42 No.1 (JANUARY 2015)
Research Article View: 1,389 Download: 300
Exact Solution for Average Run Length of CUSUM Charts for MA(1) Process
page: 1449 - 1456

Kanita Petcharat[a], Yupaporn Areepong*[a], Saowanit Sukparungsee[a] and Gabriel Mititelu[b]

Vol.41 No.5/2 (OCTOBER 2014)
Research Article View: 1,215 Download: 384
Effect of Morinda citrifolia Linn. leaf extracts on in vitro lipase activity
page: 1182 - 1193

Aurasorn Saraphanchotiwitthaya*[a], Pattana Sripalakit [b]

Vol.41 No.5/1 (OCTOBER 2014)
Research Article View: 1,358 Download: 427
Effect of Fermented Red Dragon Fruit (Hylocereus polyrhizus) Liquid on Cholesterol and Triglycerides Level of Golden Syrian Hamsters
page: 1084 - 1093

Foong Jia Huan*, Hon Wei Min and Ho Chun Wai

Vol.41 No.5/1 (OCTOBER 2014)
Research Article View: 1,203 Download: 502
An Agent Model for Information Filtering using Revolutionary RSVD Technique
page: 1429 - 1438

Dussadee Praserttitipong [a] * and Peraphon Sophatsathit [b]

Vol.41 No.5/2 (OCTOBER 2014)
Research Article View: 906 Download: 272
Optimization of Solid-state Fermentation for Fruiting Body Growth and Cordecepin Production by Cordyceps militaris
page: 858 - 872

Ting-chi Wen, Cuang-rong Li, Ji-chuan Kang*,Chao Kang and Kevin D. Hyde

Vol.41 No.4 (SPECIAL ISSUE 1)
Research Article View: 1,277 Download: 583
Phenotypic Marking of Cordyceps militaris Fruiting-Bodies and Their Cordycepin Production
page: 846 - 857

Ting-chi Wen, Ji-chuan Kang*, Kevin D. Hyde, Guang-rong Li, Chao Kang and Xu Chen

Vol.41 No.4 (SPECIAL ISSUE 1)
Research Article View: 1,124 Download: 323
Modeling Incidence Rates of Terrorism Injuries in Southern Thailand
page: 743 - 749

Sumpunt Khongmark*[a] and Metta Kuning [b]

Vol.40 No.4 (OCTOBER 2013)
Research Article View: 801 Download: 309
Logistic Models and Algorithms for a Biomass Transportation System
page: 459 - 470

Pidchaporn Panyoyai, Chulin Likasiri*, Pachara Tinamas and Wetchayan Rangsri

Vol.40 No.3 (JULY 2013)
Research Article View: 969 Download: 345
Theoretical Investigation of Superconducting State Parameters of (Ni33Zr67)1-xCox Bulk Metallic Glasses
page: 507 - 516

Aditya M. Vora*

Vol.40 No.3 (JULY 2013)
Research Article View: 832 Download: 530
Optimal Rain Gauge Network Design and Spatial Precipitation Mapping based on Geostatistical Analysis from Co-located Elevation and Humidity Data
page: 187 - 197

Aksara Putthividhya *[a] and Kenji Tanaka [b]

Vol.40 No.2 (APRIL 2013)
Research Article View: 832 Download: 296
Comparative Ultrasonic Irradiation Efficiency of Microcystis aeruginosa and M. wesenbergii from Surface Bloom and Re-flotation Behavior
page: 731 - 735

Pawalee Srisuksomwong[a], Yuwadee Peerapornpisal*[a], Nakao Nomura [b] and Niwooti Whangchai [c]

Vol.39 No.4 (OCTOBER 2012)
Research Article View: 903 Download: 266
Evaluation of Precipitation Simulations over Thailand using a WRF Regional Climate Model
page: 623 - 638

Chakrit Chotamonsak *[a], Eric P. Salathé Jr. [b], Jiemjai Kreasuwan [c] and Somporn Chantara [a]

Vol.39 No.4 (OCTOBER 2012)
Research Article View: 934 Download: 292
EFFECT OF DEGREE OF ACETYLATION ON IN VITRO BIOCOMPATIBILITY OF ELECTROSPUN CELLULOSE ACETATE-BASED FIBROUS MATRICES
page: 209 - 223

Pitt Supaphol,1,2* Artphop Neamnark,1,2 Pattama Taepaiboon,1,2 and Prasit Pavasant 3

Vol.39 No.2 (APRIL 2012)
Research Article View: 964 Download: 229
Analysis of Average Run Length for CUSUM Procedure with Negative Exponential Data
page: 200 - 208

Jaruchat Busaba, Saowanit Sukparungsee, Yapaporn Areepong and Gabriel Mititelu

Vol.39 No.2 (APRIL 2012)
Research Article View: 985 Download: 252
Comparison and Analysis of Remote Sensing-based and Ground-based Precipitation Data Over India
page: 541 - 550

Thitikon Chanyatham and Sukrit Kirtsaeng

Vol.38 No.4 (OCTOBER 2011)
Research Article View: 940 Download: 244
Water Quality and Trophic Status in Main Rivers of Thailand
page: 280 - 294

Pongpan Leelahakriengkrai*[a] and Yuwadee Peerapornpisal [a,b]

Vol.38 No.2 (APRIL 2011)
Research Article View: 962 Download: 472
Almost Primal Ideals in Commutative Rings
page: 161 - 165

Ahmad Y. Darani

Vol.38 No.2 (APRIL 2011)
Research Article View: 894 Download: 263
Wellness, Social Networking, and Algorithms
page: 17 - 30

Raymond Greenlaw

Vol.38 (SPECIAL ISSUE 2011)
Research Article View: 973 Download: 251
Self-Diffusion Coefficient of Liquid Alkali Metals
page: 125 - 129

Sant K. Srivastava* [a] and Kishor G. Kolhe [b]

Vol.31 No.2 May 2004
Research Article View: 1,032 Download: 220
Effects of Additives in Froth Flotation of Silicate Zinc Ore; A Study by Zeta Potential Measurement and Infrared Spectroscopy
page: 191 - 200

Jutarat Pomjaturad, Chairoj Rattanakawin, Nimit Sriprang, Suwalak Tongteeka, and Torranin Chairuangs

Vol.34 No.2 (MAY 2007)
Research Article View: 973 Download: 376
Microarray and RNA Interference: the Tools to Understand Gene Expression in Preimplantation Embryo Development
page: 367 - 388

Korakot Nganvongpanit, Ashraf El-Sayed , and Nguyen T. Ngu

Vol.34 No.3 (SEPTEMBER 2007)
Research Article View: 1,053 Download: 247
Population Dynamics of Polynemus paradiseus from Estuarine Set Bag Net Fishery of Bangladesh
page: 355 - 365

Md. Rashed-Un-Nabi, Md. Azharul Hoque , Ridzwan A. Rahman, Saleem Mustafa ,and Md. Abdul Kader

Vol.34 No.3 (SEPTEMBER 2007)
Research Article View: 912 Download: 345
Review of Angkak Production (Monascus purpureus)
page: 319 - 328

Patcharee Pattanagul, Renu Pinthong , Aphirak Phianmongkhol, Noppol Leksawasdi

Vol.34 No.3 (SEPTEMBER 2007)
Research Article View: 4,939 Download: 4,238
Nonlinear Model-Based Control for Parabolic Partial Differential Equations Systems
page: 221 - 228

Atthasit Tawai and Chanin Panjapornpon

Vol.35 No.1 (JANUARY 2008)
Research Article View: 833 Download: 873
Estimation of Water Content in PEM Fuel Cell
page: 212 - 220

Chinnavat Thawornkuno and Chanin Panjapornpon

Vol.35 No.1 (JANUARY 2008)
Research Article View: 975 Download: 978
Preparation of Nanosilica Powder from Rice Husk Ash by Precipitation Method
page: 206 - 211

Nittaya Thuadaij and Apinon Nuntiya

Vol.35 No.1 (JANUARY 2008)
Research Article View: 2,086 Download: 447
3-D Simulation of Particle and Gas Flow Behavior in a Riser with Venturi Pipe Inlet
page: 131 - 140

Parinya Khongprom, Sunun Limtrakul and Terdthai Vatanatham

Vol.35 No.1 (JANUARY 2008)
Research Article View: 948 Download: 396
Hydrodynamics of Mixture Between PVC Resin and Anti-static Powder in Circulating Fluidized Bed
page: 29 - 34

Thiti Saichua and Suchaya Nitivattananon

Vol.35 No.1 (JANUARY 2008)
Research Article View: 864 Download: 356
The Response of Aluminium Foams Under Quasi-Static Loading
page: 241 - 249

Mohamad R. Said and Chee-Fai Tan

Vol.35 No.2 (MAY 2008)
Research Article View: 960 Download: 256
Screening of Nutrient Parameters for Red Pigment Production by Monascus purpureus MTCC 369 Under Submerged Fermentation Using Plackett- Burman Design
page: 104 - 109

Md. Makhmur Ahmad, Md. Shivli Nomani, and Bibhu P. Panda

Vol.36 No.1 (JANUARY 2009)
Research Article View: 928 Download: 388
What Analytical Chemists Do : A Personal Perspective
page: 81 - 92

Gary D. Christian

Vol.32 No.2 (MAY 2005)
Research Article View: 3,191 Download: 525
Microstructural Restoration by HIP and Heat Treatment Processes in Cast Nickel Based Superalloy, IN-738
page: 287 - 295

Panyawat Wangyao, Gobboon Lothongkum, Viyaporn Krongtong, Weerasak Homkrajai and Nutthita Chuankrerk

Vol.36 No.3 (SEPTEMBER 2009)
Research Article View: 1,020 Download: 279
Effect of Hardness Test on Precipitation Hardening Aluminium Alloy 6061-T6
page: 276 - 286

Chee Fai Tan, and Mohamad R. Said

Vol.36 No.3 (SEPTEMBER 2009)
Research Article View: 1,515 Download: 1,018
On Preliminary-prediction Intervals for the Difference Between Two Means with Missing Data
page: 21 - 28

Sa-aat Niwitpong, Pawat Paksaranuwat, and Suparat Niwitpong

Vol.37 No.1 (JANUARY 2010)
Research Article View: 908 Download: 280
Characterization of Nitrate Route Synthesized Lead Zirconate Titanate Powders and Ceramics
page: 129 - 140

Suwimon Nualpralaksana and Sukon Phanichphant*

Vol.29 No.3 (DECEMBER 2002)
Opinion View: 1,342 Download: 312
Study of Crack Growth Behavior in Commercially As-Received Lead-Zirconate-Titanate
page: 21 - 29

Sukanda Jiansirisomboon

Vol.29 No.1 (APRIL 2002)
Opinion View: 938 Download: 261
Determination of Number Density and Volume Fraction of Copper Precipitates Within Proeutectoid Cementite in High-Carbon Copper-Containing Steels Using Transmission Electron Microscopy
page: 31 - 38

Torranin Chairuangsri [a], Rik M. D. Brydson [b], David V. Edmonds [b]

Vol.28 No.1 (JUNE 2001)
Opinion View: 1,102 Download: 816
Selection of Starter Cultures for the Production of Vegetarian Kapi, a Thai Fermented Condiment
page: 314 - 325

Suttida Wittanalai, Nopakarn Chandet, and Nuansri Rakariyatham

Vol.37 No.2 (MAY 2010)
Research Article View: 981 Download: 302
Age Hardening and Precipitation Behavior of an Experimental Cast Al-Mg-Si Alloy Treated by T6 and T6I6 Heat Treatments
page: 269 - 281

Suttawan Imurai, Julatep Kajornchaiyakul, Chachana Thanachayanont, John T.H. Pearce, and Torranin Ch

Vol.37 No.2 (MAY 2010)
Research Article View: 1,016 Download: 293
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