Chiang Mai Journal of Science

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In vitro

-1 benzyladenine (BA) to produce multiple shoots.
This research revealed that the medium containing 2 mg L-1 BA induced shoot formation at a rate of 100% with the highest number of 9.8 shoots per explant. Then, the elongated shoots were separated and cultured on MS solid and in liquid media supplemented with 0, 1, 2, 3 and 4 mg L-1 naphthalene acetic acid (NAA) to induce roots. It was found that the solid medium containing 1 mg L-1 NAA induced 100% root formation with the highest number of 21.5 roots per explant. For the Stemona alkaloids analysis from the in vitro roots, HPLC analysis showed the content of oxyprotostemonine 90 mg g-1 DW, stemocurtisine 7 mg g-1 DW and stemocurtisinol 9 mg g-1 DW. propagation of Stemona curtisii was explored for multiple shoot induction and root regeneration in different media. Shoot explants were cultured on solid MS media supplemented with 0, 1, 2 and 3 mg L

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