Effects of BA and NAA on Micropropagation and Stemona Alkaloids Production of Stemona curtisii Hook.f.
Jiraporn Palee* [a], Srisulak Dheeranupattana [a], Araya Jatisatienr [a] and Sunanta Wangkarn [b]* Author for corresponding; e-mail address: jik_jaja@hotmail.com
Volume: Vol.40 No.3 (JULY 2013)
Research Article
DOI:
Received: 6 March 2012, Revised: -, Accepted: 3 October 2012, Published: -
Citation: Palee J., Dheeranupattana S., Jatisatienr A. and Wangkarn S., Effects of BA and NAA on Micropropagation and Stemona Alkaloids Production of Stemona curtisii Hook.f. , Chiang Mai Journal of Science, 2013; 40(3): 356-363.
Abstract
In vitro -1 benzyladenine (BA) to produce multiple shoots. This research revealed that the medium containing 2 mg L-1 BA induced shoot formation at a rate of 100% with the highest number of 9.8 shoots per explant. Then, the elongated shoots were separated and cultured on MS solid and in liquid media supplemented with 0, 1, 2, 3 and 4 mg L-1 naphthalene acetic acid (NAA) to induce roots. It was found that the solid medium containing 1 mg L-1 NAA induced 100% root formation with the highest number of 21.5 roots per explant. For the Stemona alkaloids analysis from the in vitro roots, HPLC analysis showed the content of oxyprotostemonine 90 mg g-1 DW, stemocurtisine 7 mg g-1 DW and stemocurtisinol 9 mg g-1 DW. propagation of Stemona curtisii was explored for multiple shoot induction and root regeneration in different media. Shoot explants were cultured on solid MS media supplemented with 0, 1, 2 and 3 mg L