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Effects of BA and NAA on Micropropagation and Stemona Alkaloids Production of Stemona curtisii Hook.f.
Paper Type  |
Contributed Paper |
Title |
Effects of BA and NAA on Micropropagation and Stemona Alkaloids Production of Stemona curtisii Hook.f. |
Author |
Jiraporn Palee* [a], Srisulak Dheeranupattana [a], Araya Jatisatienr [a] and Sunanta Wangkarn [b] |
Email |
jik_jaja@hotmail.com |
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Abstract: In vitro -1 benzyladenine (BA) to produce multiple shoots.This research revealed that the medium containing 2 mg L-1 BA induced shoot formation at a rate of 100% with the highest number of 9.8 shoots per explant. Then, the elongated shoots were separated and cultured on MS solid and in liquid media supplemented with 0, 1, 2, 3 and 4 mg L-1 naphthalene acetic acid (NAA) to induce roots. It was found that the solid medium containing 1 mg L-1 NAA induced 100% root formation with the highest number of 21.5 roots per explant. For the Stemona alkaloids analysis from the in vitro roots, HPLC analysis showed the content of oxyprotostemonine 90 mg g-1 DW, stemocurtisine 7 mg g-1 DW and stemocurtisinol 9 mg g-1 DW. propagation of Stemona curtisii was explored for multiple shoot induction and root regeneration in different media. Shoot explants were cultured on solid MS media supplemented with 0, 1, 2 and 3 mg L |
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Start & End Page |
356 - 363 |
Received Date |
2012-03-06 |
Revised Date |
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Accepted Date |
2012-10-03 |
Full Text |
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Keyword |
Benzyladenine, Naphthalene acetic acid, Stemona alkaloids and Stemona curtisii |
Volume |
Vol.40 No.3 (JULY 2013) |
DOI |
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Citation |
[a] J.P., [a] S.D., [a] A.J. and [b] S.W., Effects of BA and NAA on Micropropagation and Stemona Alkaloids Production of Stemona curtisii Hook.f. , Chiang Mai Journal of Science, 2013; 40(3): 356-363. |
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