A Poly(pyrrole-3-carboxylic acid) Thin Film Modified Screen Printed Carbon Electrode as Highly Sensitive and Selective Label-free Electrochemical Immunosensing Platform
Supakiet Chanarsa, Chammari Pothipor, Nawee Kungwan and Kontad Ounnunkad* Author for corresponding; e-mail address: kontad.ounnunkad@cmu.ac.th, suriyacmu@yahoo.com
Volume: Vol.47 No.3 (May 2020)
Research Article
DOI:
Received: 23 July 2019, Revised: -, Accepted: 23 October 2019, Published: -
Citation: Chanarsa S., Pothipor C., Kungwan N. and Ounnunkad K., A Poly(pyrrole-3-carboxylic acid) Thin Film Modified Screen Printed Carbon Electrode as Highly Sensitive and Selective Label-free Electrochemical Immunosensing Platform, Chiang Mai Journal of Science, 2020; 47(3): 530-541.
Abstract
Giving good stability and sensitivity of the low-cost electrochemical immunosensor is achieved using a conducting poly(pyrrole-3-carboxylic acid) (PP3C) film on a screen-printed carbon electrode (SPCE). The PP3C film offers high density surface carboxylic groups, on which can be covalently conjugated with capturing antibodies, and improves electrode reactivity. Our simple immunosensor exhibitessatisfactory sensitivity, selectivity, stability, reproducibility, and regenerability for the detection of human immunoglobulin G (IgG). The proposed sensitive immunosensor provides a detection limit of 0.120 ng mL-1 and a sensitivity of 3.61 μA cm-2 mL ng-1, which thus is promising for clinical applications.