Chiang Mai Journal of Science

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A high-performance liquid chromatographic (HPLC) method with photo-diode array detection has been developed for the simultaneous determination of the main phenolics classes including, catechin, epicatechin, epigallocatechin gallate, gallic acid, quercetin and rutin. Phenolic compounds were separated within 35 min, using an octadecylsilyl column and a gradient elution system of acetonitrile-acetic acid solution (pH 3.0)-methanol as the mobile phase with flow rate of 1.0 mL/min. The detection limits and quantitation limits of these compounds were in the range of 0.15-0.46 and 0.42-2.47 µg/mL, respectively. The recovery tests of all compounds range of 96–-103% were obtained. This method was applied to the analysis of phenolic compounds in broccoli seeds genotypes cultivated in Thailand.

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