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Preparation of Magnetic Graphene Oxide and its Application in the Detection of Salmonella in Chicken


Paper Type 
Contributed Paper
Title 
Preparation of Magnetic Graphene Oxide and its Application in the Detection of Salmonella in Chicken
Author 
Lili Liu, Mengjun Zhang, Zhengdi Huang, Weiwei Cheng, Yuanyuan Meng, Haoyang Wang and Yuanyuan Li
Email 
yangliuyilang@126.com
Abstract:

This study aimed to investigate the quenching effect of magnetic graphene oxide (Fe3O4/GO) on fluorescence. The structure of Fe3O4/GO prepared by chemical co-precipitation was characterized, and a new Fe3O4/ GO-fluorescence labeling method was established for the rapid detection of Salmonella in chicken. Fe3O4/GO and GO were characterized by scanning electron microscopy, transmission electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. Results confirmed the chemical structure formation of the nanocomposite. The detection method was used to detect Salmonella target DNA and the Salmonella in artificial simulated contaminated chicken. The assay had a linear response between the target DNA concentration of Salmonella and fluorescence intensity in the 0.005–1 pmol/L concentration range, and the minimum limit of detection was 5 fmol/L (S/N=3). Under optimal conditions, the minimum detection limit for Salmonella-contaminated chicken samples was 102 CFU/mL (S/N=3). The detection method can realize the rapid, selective, and sensitive detection of Salmonella, only requiring 5 h for the entire process. This study may serve as a basis for the detection of Salmonella and other pathogenic bacteria.

Start & End Page 
1524 - 1537
Received Date 
2021-01-11
Revised Date 
2021-06-11
Accepted Date 
2021-07-03
Full Text 
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Correspondence & Author Name 
Keyword 
magnetic graphene oxide, Salmonella, fluorescent mark, rapid test, chicken
Volume 
Vol.48 No.6 (November 2021)
DOI 
View:319 Download:59

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Chiang Mai Journal of Science

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