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Detection of Staphylococcus aureus Enterotoxin Type I using qPCR


Paper Type 
Contributed Paper
Title 
Detection of Staphylococcus aureus Enterotoxin Type I using qPCR
Author 
Chotika Jackranukul [a], Nathinee Panvisavas [b], Soraya Chaturongakul*[c]
Email 
scsch@mahidol.ac.th.
Abstract:

Epidemiological surveillance reports revealed that Staphylococcus aureus ranked third among top bacterial pathogens causing food poisoning in Thailand during 2010-2012.  Thailand Food and Drug Administration (FDA) abides by the international “zero tolerance” policy for staphylococcal enterotoxins (SEs).  Specifically, SEs must be absent in 25 g of foods, while the bacteria are tolerated at low level depending on the types of foods.  Increasing numbers of S. aureus isolates from foods carried the newly described staphylococcal enterotoxin type I (SEI) and type G (SEG) rather than the classic types (e.g., SEA and SEB) previously identified as the common cause of staphylococcal food poisoning.  In this study, we developed a quantitative PCR (qPCR) for detection of SEI-encoding gene as SEI is the most prevalent enterotoxin in newly described SE subgroup 3 and is increasingly found in S. aureus isolates from food samples causing food poisoning.  The designed sei primer and probe set are specific to S. aureus sei gene and can detect as low as 150 femtogram of the target (approximately 50 bacterial cell/ml of milk).  We proposed that our sei primer and probe set can be used in multiplex qPCR assay along with the already developed primer and probe sets targeting classic sea and seb genes for enterotoxin detection in food safety surveillance.

Start & End Page 
143 - 150
Received Date 
2012-04-17
Revised Date 
Accepted Date 
2012-10-01
Full Text 
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Keyword 
Staphylococcus aureus, enterotoxin, SEI
Volume 
Vol.40 No.2 (APRIL 2013)
DOI 
Citation 
Jackranukul C., Panvisavas N. and Chaturongakul S., Detection of Staphylococcus aureus Enterotoxin Type I using qPCR, Chiang Mai J. Sci., 2013; 40(2): 143-150.
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