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α-Galactosidase from Ling-Zhi Mushroom (Ganoderma lucidum)
Paper Type  |
Contributed Paper |
Title |
α-Galactosidase from Ling-Zhi Mushroom (Ganoderma lucidum) |
Author |
Thida Sripuan* and Dararat Tongkao |
Email |
thidach@yahoo.com |
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Abstract: Ling-Zhi mushroom (Ganoderma lucidum), a famous medicinal mushroom, was found to produce α-galactosidase. Fruiting bodies of G. Lucidum strain L6 were extracted with phosphate-buffered saline in the ratio of 1g :5 ml then the crude extract was assayed for α-galactosidase activity by hydrolysis of p-nitrophenyl-α-D-galactopyranoside. During development of the fruiting body, the hightest activity of the α-galactosidase was obtained in the second week in an amount of 402 mU/ml with specific activity of 202 mU/mg protein. Then the enzyme activity gradually decreased to half of this amount by the seventh week. The enzyme showed optimum activity at pH 6.0 and was stable between pH 4 and 8. The optimum temperature of the enzyme was 70 °C and half of the enzyme activity remained after 1 hour at 78 °C . The α-galactosidase activity was strongly inhibited by Ag+, Hg++, p-chloromercuribenzoate and galactose. When p-nitrophenyl-α-D-galactopyranoside was incubated with the enzyme, two oligosaccharides were detected by HPLC. This indicated the enzymen catalyzed a transgalactosylation reaction.
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Start & End Page |
53 - 62 |
Received Date |
2003-02-21 |
Revised Date |
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Accepted Date |
2004-01-09 |
Full Text |
Download |
Keyword |
α-Galactosidase, Ling-Zhi mushroom, Ganoderma lucidum, transgalactosylation |
Volume |
Vol.31 No.1 (JANUARY 2004) |
DOI |
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Citation |
Sripuan* T. and Tongkao D., α-Galactosidase from Ling-Zhi Mushroom (Ganoderma lucidum), Chiang Mai Journal of Science, 2004; 31(1): 53-62. |
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