Chiang Mai Journal of Science

Paper Type 

Title 

Author 

Email 

Chloramphenicol adulterated in animal supplies is one of essential global concerns. In this study, the competitive ELISA for measuring the chloramphenicol level was established. The immunogen, chloramphenicol conjugated bovine serum albumin, was constructed with a modified chemical cross-linking procedure. A rabbit was injected for hyperimmune serum production and the presence of anti-chloramphenicol antibodies was demonstrated by indirect ELISA and Western blotting. The suitable dilution of serum containing anti-chloramphenicol antibodies was directly used in the assay. The interference of anti-bovine serum albumin antibodies appeared in the hyperimmune serum was prevented by coating the ELISA well with ovalbumin-chloramphenicol. In addition, the antibody populations, which reacted with bovine serum albumin, were neutralized after diluting in buffer containing bovine serum albumin. The working range of the assay was 10-1,280 ng/ml.

Start & End Page 

Received Date 

Revised Date 

Accepted Date 

Full Text 

Keyword 

Volume 

DOI 

Citation 

SDGs