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Effect of L -cysteine, P otassium Metabisulfite, Ascorbic Acid and Citric Acid on Inhibition of Enzymatic Browning in Longan


Paper Type 
Contributed Paper
Title 
Effect of L -cysteine, P otassium Metabisulfite, Ascorbic Acid and Citric Acid on Inhibition of Enzymatic Browning in Longan
Author 
Nutkridta Pongsakul, Bundit Leelasart and Nuansri Rakariyatham*
Email 
nuansri1@yahoo.com
Abstract:
The effect of different anti-browning agents on partially purified longan polyphenol oxidase (PPO) activity was investigated by spectrophotometry, using catechol as a phenolic substrate. For this purpose, L-cysteine, potassium metabisulfite, ascorbic acid, and citric acid were used to inhibit the activity of longan PPO at different concentrations (0-10 mM). Lcysteine was found to be the most potent anti-browning agent (L-cysteine > potassium metabisulfite > ascorbic acid > citric acid). Pre-incubation of PPO with some anti-browning agents increased the extent of inhibition.
Start & End Page 
137 - 141
Received Date 
2005-04-12
Revised Date 
Accepted Date 
2005-09-25
Full Text 
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Keyword 
enzymatic browning, longan, polyphenol oxidase
Volume 
Vol.33 No.1 (JANUARY 2006)
DOI 
Citation 
Pongsakul N.., Leelasart B.. and Rakariyatham* N.., Effect of L -cysteine, P otassium Metabisulfite, Ascorbic Acid and Citric Acid on Inhibition of Enzymatic Browning in Longan, Chiang Mai Journal of Science, 2006; 33(1): 137-141.
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