Paper Type |
Contributed Paper |
Title |
Rapid Screening of Antioxidant Compounds in Homemade Fruit Fermented Juice Using an On Line LC-ESI-MS/MS and DPPH Assay |
Author |
Nitra Nuengchamnong*, Saowanit Boonpathanasak, and Plubplueng Tepwitukkij |
Email |
nitra_nuengchamnong@yahoo.com |
Abstract: Fruit fermented juice used for health promotion was investigated for antioxidant compound using an on line LC-ESI-MS/MS coupled to DPPH assay. The structural elucidation of the active compounds was achieved by negative ionization LC-ESI-MS/MS. Based on their mass spectra and fragmentation patterns related to antioxidant activity trace, nine compounds showing strong DPPH scavenging were identifi ed as; HHDP-glucoside, Mucic acid, Monogalloyl glucoside, Monogalloyl diglucoside, Gallic acid, Galloyl quinic acid, Digalloyl glucoside, Gallic derivative, Ellagic rhamnoside . In addition, the confi rmation of the active compounds was performed using LC-MS/MS in multiple reaction monitoring (MRM) mode. Hydrolysable tannins were found as a major component responsible for the antioxidant activity of this fruit juice.
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Start & End Page |
430 - 438 |
Received Date |
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Revised Date |
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Accepted Date |
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Full Text |
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Keyword |
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Volume |
Vol.38 No.3 (JULY 2011) |
DOI |
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Citation |
Nuengchamnong N., Boonpathanasak S. and Tepwitukkij P., Rapid Screening of Antioxidant Compounds in Homemade Fruit Fermented Juice Using an On Line LC-ESI-MS/MS and DPPH Assay, Chiang Mai J. Sci., 2011; 38(3): 430-438. |
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