Paper Type |
Contributed Paper |
Title |
Culture Conditions and Some Properties of Pure Culture of Ectomycorrhizal Fungus, Scleroderma sinnamariense |
Author |
Jariwat Siri-in, Jaturong Kumla, Nakarin Suwannarach and Saisamorn Lumyong* |
Email |
saisamorn.l@cmu.ac.th |
Abstract: Scleroderma are ectomycorrhizal fungi which have a worldwide distribution. In 2010, basidiomes of Scleroderma sp. CMUS01 were collected from northern Thailand and pure cultures were obtained. Samples were identified based on morphology and molecular characteristics as Scleroderma sinnamariense. In this paper we report the optimum in vitro culture conditions of S. sinnamariense. Among ten culture media tested, fungal-host agar was the best medium for the mycelia growth and biomass yield. The fungus was able to grow at temperatures ranging from 20–35˚C, with an optimal growth temperature of 30˚C. The optimal pH for mycelia growth was 5.0. In addition, this strain produced indole-3-acetic acid and siderophore in pure culture. This report provides valuable information for S. sinnamariense mycelia cultivation in Thailand.
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Start & End Page |
275 - 285 |
Received Date |
2012-09-14 |
Revised Date |
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Accepted Date |
2013-04-22 |
Full Text |
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Keyword |
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Volume |
Vol.41 No.2 (APRIL 2014) |
DOI |
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Citation |
Siri-in J., Kumla J., Suwannarach N. and Lumyong S., Culture Conditions and Some Properties of Pure Culture of Ectomycorrhizal Fungus, Scleroderma sinnamariense , Chiang Mai J. Sci., 2014; 41(2): 275-285. |
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