Chiang Mai Journal of Science

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Different extracts of anise (Pimpinella anisum L.) seed were investigated for their antimicrobial activity on several microorganisms including bacteria, yeast, dermatophytic fungi, and plant pathogenic fungi by using disc diffusion method. All extracts (acetone, aqueous, chloroform, and ethanolic extracts) showed strong antibacterial activity against the bacterium Vibrio cholerae, while  Pseudomonas  aeruginosa  showed  resistance  to  all  types  of   the  extracts. Among various extracts,  ethanolic  extract  was  found  to  show  the  strongest  and  broadest  spectrum  of antimicrobial activity as compared to the other extracts. The most sensitive microbial strain to the ethanolic extract of anise seed was the yeast Candida albicans, with MIC of 7.81 mg/ml. Bioautographic assay of the ethanolic extract demonstrated the presence of two compounds which are active constituents responsible for antimicrobial activity against the test strains.

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