Paper Type |
Contributed Paper |
Title |
Antimicrobial Activity of Anise (Pimpinella anisum L.) Seed Extracts |
Author |
Anchana Chanwitheesuk [a], Aphiwat Teerawutgulrag [a], Teerapol Wongchanapiboon [a], Jeremy D. Kilb |
Email |
nuansri1@yahoo.com |
Abstract: Different extracts of anise (Pimpinella anisum L.) seed were investigated for their antimicrobial activity on several microorganisms including bacteria, yeast, dermatophytic fungi, and plant pathogenic fungi by using disc diffusion method. All extracts (acetone, aqueous, chloroform, and ethanolic extracts) showed strong antibacterial activity against the bacterium Vibrio cholerae, while Pseudomonas aeruginosa showed resistance to all types of the extracts. Among various extracts, ethanolic extract was found to show the strongest and broadest spectrum of antimicrobial activity as compared to the other extracts. The most sensitive microbial strain to the ethanolic extract of anise seed was the yeast Candida albicans, with MIC of 7.81 mg/ml. Bioautographic assay of the ethanolic extract demonstrated the presence of two compounds which are active constituents responsible for antimicrobial activity against the test strains.
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Start & End Page |
53 - 59 |
Received Date |
2004-05-24 |
Revised Date |
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Accepted Date |
2004-11-03 |
Full Text |
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Keyword |
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Volume |
Vol.32 No.1 (JANUARY 2005) |
DOI |
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Citation |
Chanwitheesuk A., Teerawutgulrag A., Wongchanapiboon T. and Kilb J.D., Antimicrobial Activity of Anise (Pimpinella anisum L.) Seed Extracts, Chiang Mai J. Sci., 2005; 32(1): 53-59. |
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