Paper Type |
Contributed Paper |
Title |
Effect of Ethyl-p-Methoxy Cinnamate from Kaempferia galanga on Cytochrome P450 Enzymes Expression in Mouse Hepatocytes |
Author |
Wanna Sirisangtragul [a], Kanokwan Jarukamjorn [a], Nobuo Nemoto [c] Chavi Yenjai [d] and Bungorn S |
Email |
bungorn@kku.ac.th |
Abstract: Kaempferia galanga, a plant in the Zingiberaceae family, has been widely used as a spice, food flavoring and folk medicine. Its rhizome contains ethyl-p-methoxycinnamate (EPMC) as a major constituent. This study aimed to investigate the effect of EPMC on cytochrome P450 expression in mouse primary hepatocytes. EPMC was isolated from the dichloromethane extract of dried rhizome of K. galanga by column chromatographic method. For cytotoxicity test, EPMC at a concentration of 2.42 mM is relatively non-toxic to primary cultured hepatocytes. EPMC at this concentration induced CYP1A1 and CYP1A2 mRNA expressions in the mouse hepatocytes. However, EPMC at this concentration did not show significant induction of CYP2B9 and CYP3A11 mRNA expressions. Moreover, EPMC can modulate the inductive expression of CYP1A1 mRNA by specific inducers, B[a]A and β-NF. In which the synergistic effects were observed at 72 and 48 h for B[a]A and β-NF treatments, respectively. Therefore, the consumption of K. galanga may be potentially caused herbal-drug or herbalchemical interactions.
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Start & End Page |
453 - 462 |
Received Date |
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Revised Date |
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Accepted Date |
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Full Text |
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Keyword |
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Volume |
Vol.38 No.3 (JULY 2011) |
DOI |
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Citation |
Sirisangtragul W., Jarukamjorn K. and S N.N..C.Y. .B., Effect of Ethyl-p-Methoxy Cinnamate from Kaempferia galanga on Cytochrome P450 Enzymes Expression in Mouse Hepatocytes, Chiang Mai J. Sci., 2011; 38(3): 453-462. |
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