Chiang Mai Journal of Science

A Sensitive Method for the Determination of Tranexamic Acid in Human Serum by Liquid Chromatography with Tandem Mass Spectrometer

Paper Type	Contributed Paper
Title	A Sensitive Method for the Determination of Tranexamic Acid in Human Serum by Liquid Chromatography with Tandem Mass Spectrometer
Author	Paisarn Jittorntrum [a], Saowanee Kajanachumpol* [a], Duangkamol Viroonudomphol [b], Prapin Wi
Email	raskd@mahidol.ac.th
Abstract:  Tranexamic acid (TA) is a synthetic lysine analog used for the management of bleeding disorders. In this study, we developed and validated a method for the determination of TA in human serum by liquid chromatography with Q-trap mass spectrometer. Serum sample (100 µL) was deproteinated with perchloric acid, and after pH adjustment, chromatographic separation was performed on a C18 column and isocratically eluted using a mobile phase consisting of ammonium acetate buffer (pH 3..8) /acetonitrile (95:5, v/v) at a flow rate of 200 µL /min. The total run time   was   5 minutes. Detection and quantitation were performed with the mass spectrometer using multiple reaction monitoring mode with the ion transition  m/z 158.1 to m/z  95.1 for TA and m/z 144.0 to m/z 81.1 for the internal standard (cis-4-aminocyclohexanecarboxylic acid).  The results were linear over the concentration range of 0.1-100 µg/mL of TA, with limit of quantitation of 0.03 µg/mL. The intra-day and inter-day assay coefficient of variations for serum were less than 1.8% and 2.1%, respectively, and the recovery of added standard TA was 92.5 to 99.3% . In conclusion; a simple and sensitive LC-MS/MS method has been developed for the determination of TA in human serum.  The method showed excellent linearity, sensitivity, recovery and precision.  This method is suitable for clinical pharmacokinetic studies.
Start & End Page	156 - 165
Received Date	2012-05-02
Revised Date
Accepted Date	2013-03-19
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Volume	Vol.41 No.1 (JANUARY 2014)
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