Paper Type |
Contributed Paper |
Title |
Cellulase and xylanase acting at alkaline pH from mushroom, Leucoagaricus meleagris KKU-C1 |
Author |
Santhaya Boonrung[a], Wiyada Mongkolthanaruk[a], Tadanori Aimi[b], and Sophon Boonlue *[a] |
Email |
bsopho@kku.ac.th. |
Abstract: Cellulase and xylanase production by Leucoagaricus meleagris KKU-C1 isolated from compost at pulp and paper industry, was studied in solid state fermentation using agricultural wastes as substrates. The highest cellulase and xylanase activity was obtained using a mixture of rice bran and rice husk as carbon source and sodium nitrate as nitrogen source, respectively. Crude carboxymethyl cellulase (CMCase) exhibited the highest activity at pH 8.0 and 40 ˚C, whereas crude xylanase did at pH 8.0 and 50 ˚C, respectively. Crude CMCase and crude xylanase retained over 46% and 52% of the original activity in the pH range of 7.0-11.0, respectively, after incubation at 4 ˚C for 60 min. As for thermo-stability, crude CMCase and crude xylanase retained over 50% and 69% of the original activity up to 50 ˚C, respectively, after treated at pH 8.0 for 60 min. Thus, crude CMCase and xylanase from L. meleagris KKU-C1 exhibited the highest activity at pH 8.0 and stable in alkaline pH region, suggesting that these enzymes could be used in the pulp industry.
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Start & End Page |
84 - 96 |
Received Date |
2013-05-20 |
Revised Date |
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Accepted Date |
2013-08-20 |
Full Text |
Download |
Keyword |
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Volume |
Vol.41 No.1 (JANUARY 2014) |
DOI |
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Citation |
Boonrung S., Mongkolthanaruk W., Aimi T. and Boonlue S., Cellulase and xylanase acting at alkaline pH from mushroom, Leucoagaricus meleagris KKU-C1, Chiang Mai J. Sci., 2014; 41(1): 84-96. |
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