Chiang Mai Journal of Science

Evaluation of Xylanase Production by a Thermophillic Fungus Thermoascus aurantiacus SL16W using Statistic Experimental Designs and the Arabinose Inductive Effect

Paper Type	Contributed Paper
Title	Evaluation of Xylanase Production by a Thermophillic Fungus Thermoascus aurantiacus SL16W using Statistic Experimental Designs and the Arabinose Inductive Effect
Author	Niwat Chawachart [a,b], Yooth Kasinubon [b], Chartchai Khanongnuch [b], Matti Leisola [c] and Saisam
Email	saisamorn.l@cmu.ac.th
Abstract:         Different compositions of supplements, carbon and nitrogen sources were investigated to find the optimum solid substrate media for xylanase production using isolated Thermoascus aurantiacus SL16W cultured at 45°C. Corncob, ammonium phosphate and soybean meal and were selected as the carbon source, inorganic nitrogen source and supplementary organic nitrogen source, respectively, for xylanase production using solid substrate fermentation.  Applying factorial design to screen the nutritional factors affecting on xylanase production found that corncob and soybean meal were significant factors (p> 0.05). Central composite design (CCD) were applied to optimize media composition and the optimum medium composition predicted by response surface methodology (RSM) was 1.27 g corncob, 1.32 g soybean meal, 0.04 g ammonium phosphate and 50% (w/w) initial moisture content. The maximum value of xylanase activity predicted by the model was 6,377±198 U g-1 substrate. Addition 0.5% (w/w) either of L-arabinose and lactose demonstrated an inductive effect on xylanase production in solid substrate fermentation and increased the enzyme activity up to 8547±184 and 7655±187 U g-1 substrate, respectively.
Start & End Page	48 - 59
Received Date	2011-11-24
Revised Date
Accepted Date	2012-07-31
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Volume	Vol.41 No.1 (JANUARY 2014)
DOI
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