Chiang Mai Journal of Science

First determination of ethanol production and xylose reductase gene of Zygoascus meyerae E23

Paper Type	Contributed Paper
Title	First determination of ethanol production and xylose reductase gene of Zygoascus meyerae E23
Author	Wanlapa Lorliam [a], Surisa Suwannarangsee [b], Ancharida Akaracharanya [c], and Somboon Tanasupawat
Email	Somboon.T@chula.ac.th
Abstract:  Xylose presented in lignocellulosic materials is considered to be of potential economic value for alcoholic fermentation by yeasts. Screening elephant feces in Thailand identified a number of xylose fermenting yeast strains with potential for ethanol production from pentose sugar. The aim of this study was to identify the xylose fermenting yeast for ethanol production based on D1/D2 domain of large subunit ribosomal RNA gene (LSU rRNA gene) and to characterize xylose reductase gene (XYL1) of the strain. A selected strain E23 was identified as Zygoascus meyerae. This strain could ferment xylose to ethanol with a maximum yield of 3.631 g l-1. Its xylose reductase gene was isolated by PCR cloning using specific primers designed from xylose reductase-conserved regions of various yeasts.  Partial xylose reductase gene was obtained as 807 bp PCR product.  Deduced amino acid sequence of the cloned xylose reductase gene showed 58.96% identity and 73.88% similarity to xylose reductase of Ogataea siamensis (ACN78427) and tetra-amino acid motif (Ile-Pro-Lys-Ser) which was conserved among NAD(P)H-dependent xylose reductase was detected. This is apparently the first study on cloning and characterization of xylose reductase gene from Z. meyerae E23.
Start & End Page	231 - 236
Received Date	2013-03-04
Revised Date
Accepted Date	2013-04-17
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Volume	Vol.41 No.1 (JANUARY 2014)
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