Conversion of Fe-MOF to Peroxidase-like Prussian Blue Nanozymes for Hydrogen Peroxide Detection
Qing Liu, Baoquan Liu*, Yuanrui Gao, Ruofu Shi, Chengli Yang and Dali Li** Author for corresponding; e-mail address: lidali@njust.edu.cn, lbq@dlnu.edu.cn
ORCID ID: https://orcid.org/0000-0002-8297-847X
Volume: Vol.53 No.3 (May 2026)
Research Article
DOI: https://doi.org/10.12982/CMJS.2026.051
Received: 20 January 2026, Revised: 16 March 2026, Accepted: 1 May 2026, Published: -
Citation: Liu Q., Liu B., Gao Y., Shi R., Yang C. and Li D., Conversion of Fe-MOF to peroxidase-like prussian blue nanozymes for hydrogen peroxide detection. Chiang Mai Journal of Science, 2026; 53(3): e2026051. DOI 10.12982/CMJS.2026.051.
Graphical Abstract
Abstract
Compared with natural enzymes, nanozymes demonstrate potential advantages in activity, stability, storability, and cost-effectiveness. Prussian blue nanoparticles (PBNPs) exhibit exceptional catalytic activity, including efficient peroxidase-like activity, due to the presence of Fe3+/Fe2+ redox pairs. However, the application of PBNPs is limited by their insufficient stability, poor dispersibility, and relatively large average particle size. In this study, a novel PBNPs was prepared from Fe-BTC MOF and exhibited considerable peroxidase-like activity. Characterization revealed that the PBNPs exhibited a cubic morphology with distinct lattice fringes and an average particle size of 40 nm. The nanozymes exhibited excellent peroxidase-like catalytic activity, displaying optimal performance at pH 2.2 and a temperature of 40 °C along with exceptional pH and thermal stability. In addition, they showed a lower limit of detection (LOD, 1.61 μM) and a wider linear range (2.5−20 μM) in colorimetric detection compared with other nanozymes. This work developed a method for high density preparation of peroxidase-like PBNPs mediated by Fe-BTC MOF and offered an efficient method for H2O2 detection using the nanozyme.