Paper Search
Main Menu
Homepage
Submission
Papers In Press
Search
Information for Contributors
Introducing the Journal
Board of Journal of Science
Contact address
 
Select by Volumes


 
 
 
 
 
 
 
SCImago Journal & Country Rank
 
0.39
2018CiteScore
 
25th percentile
Powered by  Scopus
Home > Differentiation of Native Rhizobium leguminosarum biovar. phaseoli Isolates Using PCR-based Fingerprinting Technique
 
Differentiation of Native Rhizobium leguminosarum biovar. phaseoli Isolates Using PCR-based Fingerprinting Technique
Paper Type
Opinion
Title
Differentiation of Native Rhizobium leguminosarum biovar. phaseoli Isolates Using PCR-based Fingerprinting Technique
Author
Hataichanoke Niamsup [a] and Ampan Bhromsiri [b]
Email
-
Abstract:
To characterize the collection of 33 native isolates of Rhizobium leguminosarum biovar phaseoli and examine their genetic relatedness, ERIC primers were used to fingerprint their genomes. Primary experiments were performed with three standard strains, CIAT899, UMR1899 and TAL182, in order to find an optimal PCR condition. At a Mg2+ concentration of 2.5 mM and an annealing temperature of 48° C, differentiatable PCR patterns among the three standard strains were obtained. These conditions were then used with purified genomic DNA from native isolates. The obtained ERIC-PCR patterns indicated that 33 isolates could be classified into 11 different strains. The results have shown that ERIC-PCR could be successfully used in typing and assessment of phylogenetic relationship of rhizobium.
Start & End Page
-
Received Date
1998-02-20
Accepted Date
1998-07-22
Full Text
None
Correspondence:
Author Name
Hataichanoke Niamsup - Department of Chemistry, Faculty of Science;
Ampan Bhromsiri - Department of Soil Science, Faculty of Agriculture; Chiang Mai University, Chiang Mai 50200 Thailand.
Keyword:
Keyword
Volume
Vol.25 No.2 (DECEMBER 1998)
 




Besucherzähler-Counter.com