Paper Search
Main Menu
Homepage
Submission
Papers In Press
Search
Information for Contributors
Introducing the Journal
Board of Journal of Science
Contact address
 
Select by Volumes


 
 
 
 
 
 
 
SCImago Journal & Country Rank
 
0.39
2018CiteScore
 
25th percentile
Powered by  Scopus
Home > Optimization of Culture Conditions for Xylanase Production from Cellulase-free Xylanase-producing Thermophilic Fungus, Thermomyces dupontii KKU−CLD−E2−3
 
Optimization of Culture Conditions for Xylanase Production from Cellulase-free Xylanase-producing Thermophilic Fungus, Thermomyces dupontii KKU−CLD−E2−3
Paper Type
Contributed Paper
Title
Optimization of Culture Conditions for Xylanase Production from Cellulase-free Xylanase-producing Thermophilic Fungus, Thermomyces dupontii KKU−CLD−E2−3
Author
Wasan Seemakram, Santhaya Boonrung, Urachart Kokaew, Tadanori Aimi and Sophon Boonlue
Email
bsopho@kku.ac.th
Abstract:
The thermoalkaliphilic cellulase-free xylanase-producing fungi, Thermomyces dupontii KKU−CLD−E2−3 was isolated from elephant dung. The production of thermo-alkali-stable and cellulase-free xylanase of the fungus was optimized in solid-state fermentation using statistical approaches. Response surface methodology (RSM) using central composite design (CCD) was employed to optimize conditions. The maximum xylanase production was observed at a 1.72% nitrogen concentration, initial pH of 10.54, initial moisture content of 74.73% and under incubation for 8 days at a temperature of 43.93 ºC. The maximum xylanase activity was evaluated at pH 11.0 and retained over 80% of the original activity within a pH range of 7.0–10.0 after incubation at 4 ºC for 24 h. In addition, the optimum temperature for xylanase activity was 80 ºC. The enzyme also retained more than 80% of its residual activity after heated at 60 ºC for 90 min. Therefore, its properties demonstrate significant promise for use in pulp bleaching for the paper industry; for which this study will be the focus of future investigation.
Start & End Page
391 - 402
Received Date
2019-10-07
Accepted Date
2019-12-23
Full Text
Download
Correspondence:
Author Name
Sophon Boonlue - Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
Keyword:
Keyword
elephant dung, thermo-alkaliphilic fungi, response surface methodology,
Volume
Vol.47 No.3 (May 2020)
 




Besucherzähler-Counter.com