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Growth Inhibition of Ralstonia solanacearum PT1J by Antagonistic Bacteria Isolated from Soils in the Northern Part of Thailand


Paper Type 
Contributed Paper
Title 
Growth Inhibition of Ralstonia solanacearum PT1J by Antagonistic Bacteria Isolated from Soils in the Northern Part of Thailand
Author 
Narumol Thongwai and Jirapa Kunopakarn
Email 
nthongw@hotmail.com
Abstract:
The most serious infectious disease found in Siam Tulip or Patumma (alismatifolia isolates of the bacteria isolated from infected Patumma’s rhizomes, the isolate PT1J, which was identified as Patumma rhizomes upon re-infection. Concurrently, five of seventy-six bacterial isolates, screened for their ability to inhibit growth of minimizing growth of the pathogen; namely enterobacteria N9A and Ba3. Optimal conditions, for production of inhibitory substances from these antagonistic bacteria, were 30°C in a medium pH of 7, containing 0.25% (w/v) glucose and 2.0% (w/v) peptone. The promising time for introducing mixed cultures of these five antagonistic strains to Patummas grown in pots was at the beginning of Patumma cultivation, in order to prevent and protect the plants from CurcumaGagnep.) was a bacterial wilt caused by Ralstonia solanacearum. Among ninety-fourR. solanacearum, was selected for further study due to its high virulence againstR. solanacearum PT1J, showed high potential forBacillus sp. Ba4, N9C8 and N11IV, andR. solanacearum infection.
Start & End Page 
345 - 354
Received Date 
2007-03-15
Revised Date 
Accepted Date 
2007-06-26
Full Text 
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Keyword 
agar disc diffusion, bacterial wilt, Bacillus, Curcuma alismatifolia Gagnep, Patumma, Ralstonia solanacearum, enterobacteria
Volume 
Vol.34 No.3 (SEPTEMBER 2007)
DOI 
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Chiang Mai Journal of Science

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