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Comparative Structural Analysis of The Homeodomain Protein in the Bipolar Basidiomycete, Mycoleptodonoides aitchisonii (Bunaharitake)


Paper Type 
Contributed Paper
Title 
Comparative Structural Analysis of The Homeodomain Protein in the Bipolar Basidiomycete, Mycoleptodonoides aitchisonii (Bunaharitake)
Author 
Weitong Zhang, Rini Riffiani, Kozue Sotome, Takayuki Wada, Norihiro Shimomura, Sophon Boonlue and Tadanori Aimi
Email 
taimi@tottori-u.ac.jp
Abstract:

     Mycoleptodonoides aitchisonii is a bipolar mushroom with monokaryons can form clamp cells, regardless of the mating type. Previously, only HD2-18 was identified and characterized at the A locus of Maspi18 (A1). In this study, unlike the A locus in strain Maspi18, HD1-7-like gene (Mahd1-7) and HD2-7 gene (Mahd2-7) were identifi ed at the A locus of the compatible strain Maspi7 (A2), but the nine flanking genes were highly conserved, suggesting that recombination events may have occurred between HD1 and HD2 genes over evolutionary time. Following phylogenetic and HD protein structural comparisons with other basidiomycetes, homeodomains were identifi ed in HD2-7 and HD2-18, but the former lacked nuclear localization signals (NLS) whereas the latter, like the HD1-7-like protein, did not. These findings indicated that one homeodomain and several NLS on the same or different HD proteins is the minimum sufficient for driving clamp cell formation in M. aitchisonii. The findings implied that over evolutionary time, unlike HD2, the homeodomain in HD1 does not play a major role in clamp cell formation, and that HD1-7 performs the function of carrying the NLS. Herein, we propose a new hypothesis for clamp cell formation in M. aitchisonii. The findings of this study provide a foundation for understanding the role of heterokaryonization in basidiomycete mushrooms.

Article ID
e2024014
Received Date 
2023-03-29
Revised Date 
2023-11-08
Accepted Date 
2023-11-13
Full Text 
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Keyword 
bipolar mushroom, homeodomain genes, nuclear localization signals, monokaryotic clamp cells formation, heterodimerization
Volume 
Vol.51 No.1 (January 2024)
DOI 
https://doi.org/10.12982/CMJS.2024.014
Citation 
Zhang W., Riffiani R., Sotome K., Wada T., Shimomura N., Boonlue S., et al., Comparative Structural Analysis of The Homeodomain Protein in the Bipolar Basidiomycete, Mycoleptodonoides aitchisonii (Bunaharitake), Chiang Mai J. Sci., 2024; 51(1): e2024014. DOI 10.12982/CMJS.2024.014.
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